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hepg2 lucia ahr reporter cells  (InvivoGen)


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    InvivoGen hepg2 lucia ahr reporter cells
    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in <t>HepG2-Lucia™</t> AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.
    Hepg2 Lucia Ahr Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 42 article reviews
    hepg2 lucia ahr reporter cells - by Bioz Stars, 2026-03
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    1) Product Images from "ALDH3A1-dependent Nrf2/HO-1/GPX4 pathway supports AHR as a promising therapeutic target for ferroptosis and promotes imperatorin-mediated lung protection"

    Article Title: ALDH3A1-dependent Nrf2/HO-1/GPX4 pathway supports AHR as a promising therapeutic target for ferroptosis and promotes imperatorin-mediated lung protection

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-025-02860-8

    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in HepG2-Lucia™ AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.
    Figure Legend Snippet: A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in HepG2-Lucia™ AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.

    Techniques Used: Immunofluorescence, Translocation Assay, Western Blot, Expressing, Activity Assay, Luciferase, Reporter Assay, Control



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    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in <t>HepG2-Lucia™</t> AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.
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    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in <t>HepG2-Lucia™</t> AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.
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    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in <t>HepG2-Lucia™</t> AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.
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    Image Search Results


    A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in HepG2-Lucia™ AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.

    Journal: Cell Death Discovery

    Article Title: ALDH3A1-dependent Nrf2/HO-1/GPX4 pathway supports AHR as a promising therapeutic target for ferroptosis and promotes imperatorin-mediated lung protection

    doi: 10.1038/s41420-025-02860-8

    Figure Lengend Snippet: A Immunofluorescence showing the intracellular localization of AHR in A549 cells treated with IMP (20 μM) or Tapi (1 μM) for 24 h (scale bar = 20 μm). B Schematic diagram of AHR nuclear translocation triggered by IMP in A549 cells. C , D Western blotting analysis of AHR and CYP1A1 in the cytoplasm and nucleus of A549 cells. E mRNA expression levels of AHR, CYP1A1, and AHRR in A549 cells. F Western blotting analysis of AHR and CYP1A1 in total protein of A549 cells. Concentrations in ( E , F ): IMP, 20 μM; Tapi, 1 μM; CH, 10 μM. G Schematic diagram of compound activity using the AHR-driven luciferase reporter assay in HepG2-Lucia™ AHR reporter cells. H Effect of IMP, Tapi, and CH on AHR. I Molecular docking results of IMP with AHR. J The MST assay measured the Kd values for the interaction between AHR and IMP or Tapi. Data are expressed as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01 , *** p < 0.001, **** p < 0.0001, versus control group; ### p < 0.001, #### p < 0.0001, versus IMP group.

    Article Snippet: A549 cells were sourced from the American Type Culture Collection (VA, USA), and HepG2-Lucia AHR reporter cells were sourced from InvivoGen (Toulouse, France).

    Techniques: Immunofluorescence, Translocation Assay, Western Blot, Expressing, Activity Assay, Luciferase, Reporter Assay, Control

    Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

    Article Snippet: The HepG2-AhR Lucia™ reporter cells (InvivoGen) were used to assess the activation of the human aryl hydrocarbon receptor (AhR) by indole and its derivatives.

    Techniques: Activity Assay

    Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

    Article Snippet: The HepG2-AhR Lucia™ reporter cells (InvivoGen) were used to assess the activation of the human aryl hydrocarbon receptor (AhR) by indole and its derivatives.

    Techniques: Standard Deviation

    Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

    Article Snippet: The HepG2-AhR Lucia™ reporter cells (InvivoGen) were used to assess the activation of the human aryl hydrocarbon receptor (AhR) by indole and its derivatives.

    Techniques: Activation Assay

    Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

    Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

    Techniques: Activity Assay

    Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

    Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

    Techniques: Standard Deviation

    Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

    Journal: Toxicology Reports

    Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

    doi: 10.1016/j.toxrep.2024.101883

    Figure Lengend Snippet: Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

    Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

    Techniques: Activation Assay